FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_mdrdpm2.3510000003bf17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_mdrdpm2.3510000003bf17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10609620
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC188660.17781975226256924TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT45350.021.33555443
GATCTCG42950.021.27039740
CGTATGC47600.020.94138745
TCGTATG51700.019.4114144
ATCTCGT49100.018.74366841
AGTGATC49350.018.46459237
GTGATCT50900.017.99105538
TCTCGTA55150.016.89147642
TGATCTC56650.016.40372339
AGTTCAG48500.015.3663721
ACAGTGA109250.014.64390345
CACAGTG118800.013.44790644
GAGTTCA31600.012.896641
TGCCCCG13300.012.5189615
CAGACGT58550.012.4126315
TCAGACG58650.012.3914674
CAGTGAT77500.012.33840736
CACACAG129550.012.31463642
GTTCAGA61350.012.2144862
ACACAGT131700.012.113643
Produced by FastQC (version 0.11.2)