FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_hu.3510000003bf9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_hu.3510000003bf9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5201506
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC271330.5216373873259014TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC43200.031.3033645
CTCGTAT43250.030.53911243
TATCTCG43550.029.29539140
TCGTATG46400.029.19328344
TCTCGTA46750.027.4826742
ATCTCGT46950.027.2218241
TAGCTTA238950.022.7975445
GAGTTCA21400.019.5694791
TTATCTC66600.019.35796739
GCTTATC66350.019.2606137
CTTATCT68050.019.11044138
TGTAGGG10400.018.1855321
GTAGGGA10550.018.1309682
AGCTTAT120050.018.03072445
CTAGCTT312750.017.51167344
GACGTGT77850.017.0347861
AGTTCAG35600.016.1276191
ACTAGCT378700.014.46204443
CACTAGC409600.013.36004742
GTTCAGA45350.012.6536952
Produced by FastQC (version 0.11.2)