FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_ddpm2.3510000003bf0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_ddpm2.3510000003bf0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283740
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC270930.20395611476888287TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT58350.023.9861743
CGTATGC66450.021.50247645
AATCTCG62650.021.33421740
ATCTCGT63450.020.88791341
TCGTATG71300.020.10301844
TCTCGTA74250.018.45577242
CAATCTC80800.016.54050839
TGCCCCG28750.016.0443635
AGTTCAG68900.015.9804441
ACCAATC85600.015.66521837
GTGCCCC29200.015.4888684
CCCCGGG30500.014.9011147
CCAATCT92900.014.65233638
CCCGGGT32900.013.882498
GACCAAT102550.013.27344536
CCGGGTA40750.013.0857259
CAGACGT82700.012.9783464
TCAGACG84450.012.922563
AGACGTG85300.012.5830416
GTTCAGA90050.012.5521811
Produced by FastQC (version 0.11.2)