FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_r_90.3520000003c02d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_r_90.3520000003c02d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8237019
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1079181.3101584444566656Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG133250.041.12331445
GGTCGCC130350.040.95032544
GTGGTCG131600.039.82509242
TGGTCGC135600.038.48435643
GGTGGTC150450.034.8503341
CTCGGTG151100.034.56931738
CGGTGGT151500.034.5050340
TCGGTGG157350.033.38153539
TCTCGGT159200.032.86739337
ATCTCGG185150.028.33339736
GTGACTG40500.025.4387261
ACACTCT24700.024.5896171
TGACTGG43300.023.6374452
GACTGGA45950.023.351383
GATCTCG232500.022.5822235
GGGCGTT13300.020.6344621
CACTCTT31300.019.1167622
CTGGAGT62500.018.7517875
AGATCTC281050.018.68091234
TCAGACG65950.018.38647512
Produced by FastQC (version 0.11.2)