H7MT5BCXX_l01n02_q_r_60.3520000003bf8e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H7MT5BCXX_l01n02_q_r_60.3520000003bf8e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11607054
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1898861	16.359543084748292	Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	78461	0.6759768671706016	Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	73078	0.6295998967524404	Illumina Single End PCR Primer 1 (100% over 40bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	50367	0.43393439885779805	Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG	29097	0.2506837652344859	Illumina Single End PCR Primer 1 (100% over 42bp)
ACACTCTTTCCCTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT	22039	0.1898759151116209	Illumina Single End PCR Primer 1 (100% over 37bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	14489	0.12482926330832957	Illumina Single End PCR Primer 1 (100% over 41bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT	14283	0.12305448049091526	Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	14157	0.12196893371909875	Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	13080	0.1126900934552385	Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	12404	0.1068660488699372	Illumina Single End PCR Primer 1 (100% over 43bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACACTCT	27120	43.35532	1
GTCGCCG	210835	43.103153	45
GGTCGCC	210435	42.910484	44
GTGGTCG	210615	42.75396	42
TGGTCGC	210180	42.721455	43
CACTCTT	27510	42.552067	2
GGTGGTC	212045	42.501713	41
CGGTGGT	212785	42.345814	40
TCGGTGG	223000	40.488235	39
CTCGGTG	223255	40.375458	38
TCTCGGT	229500	39.240845	37
GTGACTG	13920	39.204067	1
TGACTGG	13965	38.948376	2
ACTCTTT	29195	38.86355	3
AGATAGA	26485	38.58051	7
CTCTTTC	27615	38.488644	4
CAGATAG	11190	37.454136	6
TCTTTCC	20815	36.527653	5
CCCTAAG	4460	35.964767	10
CTTTCAG	12735	35.454018	6