FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_r_120.3520000003c047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_r_120.3520000003c047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6759882
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1704132.520946371549089Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG81670.12081571838088298Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC80710.119395575248207Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG199650.042.26758245
GGTCGCC196600.042.22634544
TGGTCGC199000.041.26443543
GTGGTCG199100.041.24370642
GGTGGTC214150.038.4082441
CGGTGGT221150.037.1622640
CTCGGTG226300.036.3286438
TCGGTGG234600.035.10091439
ACACTCT32700.034.735771
TCTCGGT244150.033.68184337
CACTCTT35900.031.7024352
GTGACTG30700.027.1812131
GCACACG8650.027.04973618
ATCTCGG307000.026.8886236
TGACTGG32600.025.666222
GACTGGA35200.024.6011073
AGCACAC13250.023.2616717
CACACGT7950.020.94209119
GAGCACA17350.020.74691216
AGAGCAC17900.020.61186615
Produced by FastQC (version 0.11.2)