FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_d_90.3520000003bfeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_d_90.3520000003bfeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310126
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG184871.4110856513037677Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC22900.040.88061544
GTCGCCG23350.040.38035245
GTGGTCG23200.040.15798642
TGGTCGC24650.037.7957543
CTCGGTG25900.036.059938
GGTGGTC26150.035.4556241
CGGTGGT26550.035.0062140
TCTCGGT27300.034.1295437
TCGGTGG28100.033.31678839
ATCTCGG31950.028.95100636
ACACTCT3700.028.5708731
CACTCTT4800.022.4928232
GGGCGTA1003.659261E-822.4919641
GATCTCG42300.021.97198335
GAGGGTC1850.021.8840731
GCGGGTC1151.6017839E-719.558231
CGGGGAC707.000991E-419.2788261
TGACTGG4100.019.2011892
GACTGGA4850.018.0870133
GTGACTG4250.017.9935721
Produced by FastQC (version 0.11.2)