FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_d_60.3520000003bfc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_d_60.3520000003bfc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12415088
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3296892.6555510520747014Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG129290.10413941487970121Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG387400.041.9162845
GGTCGCC385050.041.82745744
GTGGTCG390800.041.09094242
TGGTCGC406600.039.3004943
GGTGGTC411600.039.1019141
CGGTGGT416250.038.63806540
CTCGGTG430250.037.3830738
TCGGTGG439200.036.67222639
TCTCGGT450650.035.71093837
ACACTCT53650.031.7794111
ATCTCGG516150.031.2443336
CACTCTT62200.027.121832
GTGACTG54350.026.3624781
TGACTGG57450.025.0183662
GATCTCG648500.024.86708635
TCGCCGT26150.023.83712645
GACTGGA63650.023.5007573
GCACACG18000.021.99833318
AGATCTC780900.020.65956934
AGATAGA85750.020.3324247
Produced by FastQC (version 0.11.2)