FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_d_30.3520000003bf4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_d_30.3520000003bf4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7859603
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG203270.25862629448332186Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC26600.038.9150444
GTCGCCG27600.038.2386345
GTGGTCG29200.034.21674342
TGGTCGC29750.033.58416443
CTCGGTG33300.029.46532438
TCTCGGT36650.026.71148537
CGGTGGT39100.025.78337740
TCGGTGG38350.025.7023139
ATCTCGG43950.022.7865436
GGTGGTC45450.022.13156941
CAGACGT26800.021.74181213
GACGTGT26050.021.59065215
CGTGTGC28650.020.88782717
AGACGTG27700.020.62930914
ACGTGTG28150.020.37971316
TCAGACG28750.019.7976412
GATCTCG52500.019.16107735
TTCCGAG11100.018.44703326
GCACACG7900.017.6565718
TCCGAGA7950.016.98213427
Produced by FastQC (version 0.11.2)