FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_d_120.3520000003c003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_d_120.3520000003c003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15492000
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12427778.02205654531371Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG569920.36788019623031243Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC546380.3526852569067906Illumina Single End PCR Primer 1 (100% over 40bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT420710.271565969532662Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG203110.13110637748515364Illumina Single End PCR Primer 1 (100% over 42bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTAG193980.12521301316808675Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1464650.042.5100145
GGTCGCC1442200.042.32244544
GTGGTCG1430850.042.0071742
TGGTCGC1445600.041.46180343
GGTGGTC1459150.041.24180241
CGGTGGT1461250.041.04098540
ACACTCT200350.040.462831
CACTCTT206850.038.9953462
TCGGTGG1575900.038.1894539
CTCGGTG1573100.038.11305638
TCTCGGT1624500.036.89261237
GTGACTG263500.035.2813841
TGACTGG271300.034.4906652
ACTCTTT233950.033.545743
CTCTTTC215650.033.1279564
GGCCGCC34450.032.98835844
GCCGCCG49900.032.9658445
ATCTCGG1863350.032.38783636
CGTCGCC35150.031.75520344
GGCGCCG36250.031.65989545