FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_q_asym.3520000003bfa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_q_asym.3520000003bfa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163553
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG483220.591923639131148Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC54250.039.57396344
GTCGCCG55850.038.96501545
GTGGTCG60000.035.66959842
CTCGGTG62100.034.75414338
TGGTCGC63550.033.570843
TCTCGGT66300.032.68890437
ATCTCGG70800.030.80137436
TCGGTGG73300.029.50522839
GGTGGTC83800.025.75393141
GATCTCG85450.025.46716935
CGGTGGT90900.023.86613740
CGGGGAC7200.023.1178421
GCGGGAC6800.022.1622541
GCGGGAG14250.021.6248841
CGGGGAG7200.021.5558241
AGATCTC101550.021.40724634
GCGGGTC7350.020.1978261
TAGATCT114100.019.21042333
GTAGATC118200.018.60118932
GCGGGTG12700.018.4194931
Produced by FastQC (version 0.11.2)