FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_201_90.3520000003bf21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_201_90.3520000003bf21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12904799
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1112940.8624233511889647Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC134150.040.20865644
GTCGCCG136100.039.9788545
GTGGTCG140800.037.97382442
TGGTCGC142400.037.34171743
CTCGGTG143550.037.24794438
GGTGGTC156100.034.36717641
TCTCGGT154900.034.3600637
TCGGTGG157150.034.26775739
CGGTGGT161700.033.0379440
ATCTCGG161500.033.01097536
GATCTCG195350.027.34815835
AGATCTC226850.023.53070634
CGGGGCG4050.022.2166651
CGGGGAC9800.021.8057691
GCGGGAC9350.021.6523361
TAGATCT257300.021.01711333
GCACACG12400.020.50224118
CGGGGAG9800.020.1990281
GTAGATC271400.020.01642232
AGTGTAG277550.019.09296629
Produced by FastQC (version 0.11.2)