FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_201_60.3520000003bef9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_201_60.3520000003bef9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800194
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG249590.21151347172766818Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC34300.034.90427844
GTCGCCG36150.033.9882645
CTCGGTG39600.030.00708638
TGGTCGC39550.029.64506543
GTGGTCG41650.028.36648242
TCTCGGT49300.024.05801837
ATCTCGG50300.023.71375536
TCGGTGG55400.022.26148239
GGTGGTC58000.020.83566941
CGGTGGT61800.019.9550740
CGGGGAG7800.019.3212471
CGGGGCG3800.017.757941
GATCTCG67700.017.61841235
GCGGGTC6400.017.5729641
GGGGACC10000.016.6448292
CGGGGAC7200.015.932821
GCACACG8300.015.44995318
CGCGGAT7000.015.4240421
GCGGGTG13450.015.2185781
GCGGGAC6150.013.89835551
Produced by FastQC (version 0.11.2)