FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_201_30.3520000003bedf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_201_30.3520000003bedf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5058407
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG174050.34408065622240364Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG23300.038.247245
GGTCGCC22550.038.02196544
GTGGTCG25150.033.01752542
TGGTCGC25600.032.52504343
CTCGGTG26950.030.39656438
TCTCGGT30450.026.90297137
GGTGGTC32750.025.56158341
TCGGTGG33400.024.99798839
CGGTGGT34550.024.1649740
ATCTCGG36350.022.96949236
GTGACTG15850.021.5709151
CGGGGAC3050.021.3871251
GACTGGA18100.020.380723
CGGGGCG1351.9754225E-919.9940951
TGACTGG17000.019.7147662
ACACTCT10450.017.650291
ACGTGTG24700.017.39657216
CGTGTGC25700.017.33242417
CAGACGT24350.017.27689213
CGGGGGT3150.017.1377941
Produced by FastQC (version 0.11.2)