FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n02_201_120.3520000003bf64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n02_201_120.3520000003bf64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810810
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2490032.538047317193993Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG114050.11624932090214773Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC107660.10973609722336891Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG283550.042.1973945
GGTCGCC281300.042.11178244
TGGTCGC281350.041.5201943
GTGGTCG285550.041.0907542
GGTGGTC296350.039.58567441
CGGTGGT303350.038.50900740
CTCGGTG338600.034.54759638
TCGGTGG348300.033.7274439
ACACTCT46250.032.876071
TCTCGGT357200.032.7682237
GTGACTG57800.028.7971021
CACTCTT53400.028.5580752
TGACTGG59200.028.3057752
ATCTCGG425000.027.64592236
GACTGGA63850.027.61853
ACTGGAG76250.023.982814
CTGGAGT88000.023.413755
AGATAGA72700.022.5587737
TCGCCGT19550.022.21565645
CCCTAAG12050.022.03059410
Produced by FastQC (version 0.11.2)