FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_r_90.3510000003c020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_r_90.3510000003c020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8237019
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC945571.147951704372662TruSeq Adapter, Index 11 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT150700.032.42749443
CATCTCG145900.032.1372940
CGTATGC155950.031.71094145
TCGTATG158450.031.28160944
ATCTCGT151300.031.27283141
TCTCGTA154800.030.58029242
ACATCTC173550.026.96498939
TACATCT184650.025.38042338
CTACATC191100.024.57087737
GCTACAT198550.023.6602636
GGCTACA243950.019.6074635
CGGCTAC280950.017.0492634
ACGGCTA319100.015.05323933
CACGGCT337400.014.21010632
TCACGGC354150.013.57613531
GTCACGG363400.013.06959830
GTATGCC42450.012.66738745
AGTCACG377950.012.65574829
GCCGTCT14850.012.27228445
TCTGAAC530300.012.0533719
Produced by FastQC (version 0.11.2)