FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_r_60.3510000003bf81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_r_60.3510000003bf81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11607054
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC174870515.065881488963523TruSeq Adapter, Index 6 (100% over 50bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA767430.661175523091389TruSeq Adapter, Index 6 (100% over 40bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT714740.6157807140382047TruSeq Adapter, Index 6 (100% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC613510.5285665079183745TruSeq Adapter, Index 6 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC512300.44136953269968415TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG456890.3936313210914673TruSeq Adapter, Index 6 (100% over 51bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT387900.334193327609228TruSeq Adapter, Index 6 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC373080.3214252298645289TruSeq Adapter, Index 6 (98% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT315320.27166238737236853TruSeq Adapter, Index 6 (100% over 51bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT267680.23061838085702024TruSeq Adapter, Index 6 (100% over 41bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC247260.21302563079313666TruSeq Adapter, Index 6 (100% over 47bp)
TAGGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC219870.18942791168198236TruSeq Adapter, Index 6 (100% over 36bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC197330.17000868609726463TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC163680.14101769492930763TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC159700.13758874560245865TruSeq Adapter, Index 6 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCAGATCGGAAGAGCACACGTC155390.13387548640680055Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA144260.1242864899224213TruSeq Adapter, Index 6 (100% over 40bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA141020.12149508393775027TruSeq Adapter, Index 6 (100% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA129920.11193193380508094TruSeq Adapter, Index 6 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC127160.10955406944776858TruSeq Adapter, Index 6 (100% over 49bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTT116930.10074046351468684TruSeq Adapter, Index 6 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2330350.037.0775945
CTCGTAT2319050.036.87018243
TATCTCG2243250.036.86031340
TCGTATG2348850.036.8238744
ATCTCGT2249450.036.77271741
TCTCGTA2252900.036.73538242
AGACGGA32550.035.900471
ATATCTC2353300.035.0981839
AATATCT2375950.034.70931238
CAATATC2432200.033.86402537
AGTCGGA21300.033.509151
GACGGAA36650.031.9914672
ACTCGTA60550.031.472942
TAACTCG60600.031.14991240
CCAATAT2742450.030.26930636
AACTCGT63000.030.0703941
TGTAGGG32300.029.5561031
TACCTCG19450.029.03498640
GCCGTCT70800.028.85493545
ACGGAAG41550.028.4895213
Produced by FastQC (version 0.11.2)