FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_r_120.3510000003c04a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_r_120.3510000003c04a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6759882
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1511012.2352609113591035TruSeq Adapter, Index 12 (100% over 50bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA78200.11568249268256459TruSeq Adapter, Index 12 (100% over 40bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA77190.1141883837617284TruSeq Adapter, Index 12 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT218400.035.28363443
ATCTCGT214700.034.93807241
CGTATGC225800.034.79490745
TCGTATG227650.034.5615644
AATCTCG218750.034.26036540
TCTCGTA219300.034.25651642
GTAATCT241400.030.67275638
TAATCTC246700.030.33321839
TGTAATC253700.029.21226937
TTGTAAT328500.022.99207536
TGCCGTC19850.018.24865245
AGTCGGA6000.017.6382641
CTTGTAA465200.016.6710835
GTATGCC46900.015.11134445
ATGCCGT27000.013.16614644
CCTTGTA591600.013.10156734
AGACGGA10350.012.6181851
GAGTGTA32450.012.5526465
TATGCCG29250.012.23028843
AGTGTAG37000.011.7997116
Produced by FastQC (version 0.11.2)