FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_d_90.3510000003bfee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_d_90.3510000003bfee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310126
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC160061.2217145526460813TruSeq Adapter, Index 9 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG23100.035.4535240
ATCTCGT24350.034.09552841
CTCGTAT25400.034.01476743
CGTATGC26450.033.34497545
TCGTATG26950.032.6428444
TCTCGTA25800.032.17930642
AGATCTC26450.030.9631939
CAGATCT28200.029.4406438
ATCAGAT37100.023.16644736
TCAGATC37600.022.20015737
GATCAGA45850.019.18701435
CGATCAG53300.016.42072734
GTATGCC5850.016.1533845
CCCCGTG2001.571243E-814.6268112
ACGATCA63750.013.87018933
CACGATC67200.013.024178532
GTGACTG1801.6849066E-512.5063221
TGCCGTC2901.0186341E-912.41343645
TCCCCGT2756.0917955E-912.2789341
TCACGAT72300.012.10546131
Produced by FastQC (version 0.11.2)