FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_d_60.3510000003bfc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_d_60.3510000003bfc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12415088
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2878572.318606199166691TruSeq Adapter, Index 8 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC130830.10537984104502522TruSeq Adapter, Index 8 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT428000.035.19986343
CGTATGC441750.034.9344245
ATCTCGT420650.034.6861941
TCGTATG447500.034.50062644
AATCTCG423600.034.36496440
TCTCGTA436200.033.43935442
GAATCTC456150.031.36992539
TGAATCT472750.030.33027838
TTGAATC487400.029.4647937
CTTGAAT575150.025.4740136
ACTTGAA718800.021.12493735
CACTTGA816800.018.58484834
TGCCGTC37200.018.26557445
GTATGCC88150.017.10101145
ACACTTG912000.016.6547233
AGACGGA21150.015.755751
ACTCGTA23250.015.6768842
CACACTT990550.015.54524832
TCACACT1033650.014.86223331
GTCACAC1043000.014.60388630
Produced by FastQC (version 0.11.2)