FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_d_30.3510000003bf4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_d_30.3510000003bf4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7859603
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC178830.22753057628992204TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32000.028.61622845
CTCGTAT31900.028.0711643
TCGTATG33450.027.30850244
ATCTCGT33400.026.13668441
AATCTCG36100.023.80791540
TCTCGTA39100.022.49910242
ACCAATC48800.017.38114737
CCAATCT52450.016.64344238
CAATCTC54350.016.0616139
GACCAAT54750.015.94426636
ACTGACC67650.013.20321333
CTGACCA72800.012.54733734
TGACCAA72700.012.53364935
CACTGAC78150.011.57321532
CGTCTGA115350.010.92263217
GTCACTG83150.010.87729130
ACGTCTG115850.010.8172316
AGTCACT85750.010.73114629
CACGTCT121200.010.39542715
ACACGTC121850.010.33997314
Produced by FastQC (version 0.11.2)