FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_d_120.3510000003c006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_d_120.3510000003c006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15492000
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC11216617.240259488768396TruSeq Adapter, Index 10 (100% over 50bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA553690.35740382132713655TruSeq Adapter, Index 10 (100% over 40bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT528600.3412083656080558TruSeq Adapter, Index 10 (100% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC506620.32702039762458046TruSeq Adapter, Index 10 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC499420.3223728375935967TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC429770.27741414923831653TruSeq Adapter, Index 10 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG357700.23089336431706686TruSeq Adapter, Index 10 (100% over 51bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCT327960.21169635941130907TruSeq Adapter, Index 10 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCAGATCGGAAGAGCACACGTC319260.2060805577072037Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG309720.19992254066615026Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT220240.14216369739220241TruSeq Adapter, Index 10 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAACTCGTATGC212560.13720630002581977TruSeq Adapter, Index 10 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG198470.1281112832429641TruSeq Adapter, Index 14 (97% over 34bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC190420.1229150529305448TruSeq Adapter, Index 10 (100% over 47bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT187750.12119158275238832TruSeq Adapter, Index 10 (100% over 41bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC177840.11479473276529821TruSeq Adapter, Index 10 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1600300.035.587345
CTCGTAT1588250.035.35015543
TCGTATG1616350.035.2506344
TATCTCG1547350.035.159140
ATCTCGT1554650.035.04176741
TCTCGTA1560900.034.90433542
TTATCTC1675150.032.35700639
CTTATCT1684500.032.1424738
GCTTATC1704700.031.58605837
GTGTGAC38650.030.1024725
AGACGGA41500.029.946181
AGTCGGA25650.029.0530051
AGCTTAT1943100.028.04190436
GAGTGTG42500.027.2166883
GACGGAA49250.025.542692
ACTCGTA46700.025.05281342
AGTGTGA46950.024.6849784
TAACTCG48150.024.20490840
GCCGTCT70400.024.033445
TGTGACT51050.023.7164136
Produced by FastQC (version 0.11.2)