FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_q_asym.3510000003bfab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_q_asym.3510000003bfab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163553
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC433950.531570016143706TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG63100.030.16519240
ATCTCGT67950.028.37651841
CTCGTAT70650.028.27929343
CGTATGC74000.027.3335345
TCGTATG77600.026.00749244
TCTCGTA75800.025.4674742
GATCATC82300.023.01837537
TCATCTC86750.022.14896839
AGATCAT92800.020.55939536
CAGATCA99500.019.74029235
ATCATCT111150.017.40810238
CCAGATC122450.016.31610934
AGTCACC123400.016.28166229
CAGTCAC133250.015.63529928
TCCAGTC135800.015.57365226
ACCAGAT129350.015.56750533
TCACCAG131250.015.32500631
CCAGTCA136650.015.31213427
ACGTCTG150850.015.21308416
CACCAGA132250.015.209125532
Produced by FastQC (version 0.11.2)