FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_201_90.3510000003bf24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_201_90.3510000003bf24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12904799
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1019150.7897449623198315TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG149800.032.03644640
CTCGTAT160550.030.97042843
CGTATGC166500.030.39068645
ATCTCGT160650.030.08283841
GCATCTC160700.029.76523639
TCGTATG171200.029.6220744
TCTCGTA166800.029.05460242
AGGCATC166250.028.83923337
GGCATCT166250.028.785138
TAGGCAT177350.027.47825836
TTAGGCA210750.023.84940135
CTTAGGC225300.022.29920834
ACTTAGG242950.020.69772133
CACTTAG271850.018.5553132
TCACTTA284600.017.63707531
AGTCACT291600.017.32170929
GTCACTT291300.017.31637830
CAGTCAC315950.016.7415728
CCAGTCA322050.016.38953427
TCCAGTC329750.016.3206826
Produced by FastQC (version 0.11.2)