FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_201_60.3510000003befc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_201_60.3510000003befc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800194
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC227490.19278496607767634TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG47100.023.3588440
CGTATGC51350.022.08285745
CTCGTAT52250.021.96084643
ATCTCGT52150.021.44200741
TCGTATG56150.020.47558444
TCTCGTA55250.020.36109242
ATGTATC64550.017.11381137
GTATCTC67950.016.29060439
CGATGTA75250.014.77004935
GATGTAT74000.014.71550536
TGTATCT77750.014.439815538
CCGATGT87850.012.60041534
ACCGATG101450.011.110846533
CCCCGGA9700.010.9102251
CACCGAT110050.010.16079832
TCACCGA111450.010.11391231
GTCACCG109750.010.10657330
AGTCACC116250.09.67695129
ACGTCTG146100.09.47079416
CGTCTGA147450.09.39934217
Produced by FastQC (version 0.11.2)