FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_201_30.3510000003bed2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_201_30.3510000003bed2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5058407
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC153160.302783069847879TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG27250.026.83442140
CGTATGC30300.025.39562245
CTCGTAT30400.024.79443
TCGTATG31900.024.40398644
ATCTCGT30950.024.28069941
TCTCGTA30900.023.95591542
CGATCTC30900.023.22731439
CACGATC31000.022.934237
ACGATCT34950.020.5353338
TCACGAT38600.019.00152636
ATCACGA57350.012.94609335
CATCACG67350.010.92366534
CCCTACG5600.010.84803540
CGGCCTT5950.010.5880645
TACGGCC6100.09.22115943
CCTACGG6550.08.9311541
ACGGCCT6351.8189894E-128.85812244
ACATCAC88050.08.40668433
ACCCCCC11350.08.32585442
TGCCGTC7203.6379788E-128.124866545
Produced by FastQC (version 0.11.2)