FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l01n01_201_120.3510000003bf67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l01n01_201_120.3510000003bf67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810810
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2208262.2508437121909406TruSeq Adapter, Index 5 (100% over 50bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA110560.11269202033267385TruSeq Adapter, Index 5 (100% over 40bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG104890.10691268101206729TruSeq Adapter, Index 5 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC317250.035.26114745
CTCGTAT309150.035.07151843
TCGTATG322000.034.7968944
ATCTCGT299250.034.69048341
GATCTCG297850.034.6344840
TCTCGTA301000.034.53364642
TGATCTC348650.029.4975739
GTGATCT347850.029.49411238
AGTGATC356800.028.70383337
CAGTGAT438750.024.04504636
ACAGTGA605650.018.20645935
CCCTACG13350.015.50506140
GCCGTCT18900.015.23754845
CACAGTG731000.015.06291734
TGCCGTC27650.015.05370945
TACGGCC14000.014.78518443
CTACGGC14800.014.5940742
ACTCGTA21750.014.48223842
AGTCGGA9550.014.1462161
CGGCCTT15300.013.82303345
Produced by FastQC (version 0.11.2)