FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28877586
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG926800.32094095399802464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1211200.065.5311412
ACGTCTG1178700.065.4636315
GCACACG1211950.065.4498311
ACACGTC1188250.065.4288413
CGTCTGA1176750.065.3733416
CACGTCT1182200.065.24888614
GAACTCC1181450.064.9981421
CTGAACT1185950.064.64741519
AGCACAC1241100.064.5242910
CTCCAGT1119300.064.4361924
TGAACTC1205450.064.29207620
AGAGCAC1259750.064.2358868
CCAGTCA1017800.063.730926
AACTCCA1207650.063.70847322
CAGTCAC928150.063.4272627
TCGGAAG1293750.062.9579623
CGGAAGA1300500.062.929874
GATCGGA1273100.062.7741851
ATCGGAA1319550.061.9167982
ACTCCAG1220250.060.8191823
Produced by FastQC (version 0.11.5)