FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513557
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT10705229.297925914641322TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG796980.6922100615821852No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCGCGTAT145650.12650304332535983TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT1238000.067.878134
CCGTACG1244700.067.8627633
CGTATGC1141450.067.6853646
GTACGTA1245600.067.63447635
GTATGCC1150050.067.4212147
CTCGTAT1172150.067.34312444
TACGTAA1250150.067.3358836
TATGCCG1122400.067.30302448
ATGCCGT1117350.067.2726649
TCGTATG1164750.067.2391145
CGTAATC1240900.067.1887938
CACCGTA1263400.067.1833231
TGCCGTC1119100.067.16833550
GCCGTCT1074700.067.0953351
GTCACCG1274850.067.0645929
ACCGTAC1263850.066.9732732
AGTCACC1286200.066.9566128
ACGTAAT1256400.066.89754537
GTAATCT1218400.066.85992439
TCACCGT1277950.066.65784530
Produced by FastQC (version 0.11.5)