FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9752445
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1361091.3956397600806771TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG807420.8279154611997299No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGGT631120.6471402812320398TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT630660.6466686046422204TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGT504380.5171831268979215TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGGT459200.47085628270654184TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT387370.39720295782237175TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTGGGGT337350.34591325559898056TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT326940.33523900929459227TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTGT260710.2673278341995264TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGAT237350.2433748665078347TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGTT145570.14926513299998104TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT137400.1408877466112344TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTGGGTT129840.13313584439594378TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTGGGAT124620.12778334048538598TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGTGT124490.1276500405795675TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTGGTGT104130.1067732245606102TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTGT103290.10591190209224458TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGTT100090.1026306736413279TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG785500.067.199728
GTCACGT776200.067.06171429
CGGAATC752700.066.47134438
CAGTCAC805050.066.03374527
ACGTCTG809350.065.9946815
CGTTTCG776300.065.94157433
CGTCTGA810600.065.9105216
GTTTCGG775550.065.8948934
ACTCCAG806500.065.8832323
GAACTCC809850.065.8088421
ACACGTC814850.065.7249813
CACGTCT814400.065.6495814
CCAGTCA809900.065.564526
GCACACG816700.065.5500611
TCCAGTC811200.065.4351725
CACACGT818550.065.4150712
ACGTTTC785500.065.41498632
CTCCAGT813550.065.275624
TCACGTT793950.065.2538530
CACGTTT795650.064.8670931
Produced by FastQC (version 0.11.5)