FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12727087
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT166596913.089947448304548TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG473240.3718368547335301No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT266400.20931734025232954TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTTT138810.10906659159319017TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA1888900.068.7661936
GCCTTAT1895100.068.45756537
CGTGGCC1911700.068.2845233
CGTATGC1777350.068.24280546
TGGCCTT1901300.068.217835
GTCACGT1952500.068.176929
AGTCACG1965200.068.1749228
TATGCCG1749850.068.1707248
ATGCCGT1742700.068.1391449
GTATGCC1787200.068.124947
CTCGTAT1807250.068.10078444
GTGGCCT1907100.068.0355334
TCGTATG1797900.068.008945
CCTTATC1898950.067.9946538
TGCCGTC1745950.067.94762450
GCCGTCT1673550.067.9210751
CTTATCT1854550.067.9005439
CAGTCAC1984650.067.8388127
TCACGTG1956000.067.79211430
TATCTCG1857750.067.75587541
Produced by FastQC (version 0.11.5)