FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10977886
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2553602.326130914458394TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC322250.064.72510533
GTCCGCA323400.064.22407534
ACGTCCG327750.064.1405932
CGTATGC326400.064.1313646
ATGCCGT324300.063.7880149
TATGCCG324950.063.71397448
GTATGCC329500.063.61123747
TCCGCAC325050.063.4341735
CTCGTAT328400.063.3369744
CACGTCC333100.063.24903531
TGCCGTC327000.063.2083950
GCCGTCT319550.063.09630251
CGCACAT325950.062.97867237
CCGCACA326900.062.7136
TCGTATG332650.062.66369245
AGTCACG348050.062.2536928
GTCACGT348150.061.89424529
TCACGTC345800.061.6971130
GCACATC337350.060.97495738
CCGTCTT332400.060.9042752
Produced by FastQC (version 0.11.5)