FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13764712
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2900352.1070909438570165TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG334700.066.37276535
CCGTCCC339500.065.6309634
CCCGTCC343200.065.3614633
GTCCCGA336600.065.23785436
CACCCGT351950.064.6442731
ACCCGTC348650.064.6016932
CGTATGC353650.064.4139446
CCCGATC336200.064.2318438
GTATGCC354450.064.22813447
TCACCCG357150.064.0285930
ATGCCGT353100.064.0258249
TATGCCG353700.064.0159248
GTCACCC359950.063.7259329
TGCCGTC356350.063.34368550
CTCGTAT355500.063.12587444
CCGATCT341500.062.95826339
GCCGTCT354450.062.954251
TCGTATG362550.062.67040645
CGATCTC347400.062.2100640
AGTCACC375300.061.9684828
Produced by FastQC (version 0.11.5)