FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13080384
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1441361.1019248364574008TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT456510.34900351549312314TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT360550.27564175485979614TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGT335600.25656739129371126TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT237180.1813249519280168TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT215860.16502573624749853TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTGT213510.16322915290560278TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT148760.11372754805975116TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCAG565000.061.1033334
ACATGTC568650.060.96116632
CATGTCA570550.060.62412333
CAGTCAC596100.059.99827
GTCACAT590650.059.68245729
CACATGT586650.059.5329131
AGTCACA599550.059.46743828
CCAGTCA598000.059.4437626
ACTCCAG598050.059.3687323
TCACATG591300.059.26690730
TCCAGTC600650.059.2193525
TGCGTGA161450.058.72609760
CTCCAGT603800.058.7008124
GAACTCC610400.058.55187221
TGTCAGA590200.058.3746535
CTCGTAT195950.058.33203544
GTCAGAA589500.058.29329336
GCGTGAA175850.058.2361261
CAGAATC586300.058.2353638
AATCTCG403600.058.22100441
Produced by FastQC (version 0.11.5)