FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_JK16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_JK16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11279402
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT7769326.888060200354593TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT264160.23419681291614575TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT143370.12710780234625912TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT120300.10665459037633378TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC826800.066.7831346
CGTATCT923500.066.7794339
TATGCCG809650.066.7305948
ATGCCGT812700.066.6527149
GTATGCC836550.066.6403947
CTCGTAT862800.066.6187944
TCGTATG849200.066.5637945
CCGTATC985350.066.4639138
TGCCGTC817150.066.3502850
GCCGTCT767400.066.2437551
GTATCTC912950.066.2081240
AGTTCCG996500.066.1019234
GTTCCGT994600.066.0997435
TCCGTAT995050.066.0938937
GTCACAG1028800.065.89870529
CAGTCAC1048000.065.6996327
TATCTCG922550.065.5749141
TTCCGTA1005350.065.4759336
CCAGTCA1049600.065.4524526
TCTCGTA883600.065.3909443
Produced by FastQC (version 0.11.5)