FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18264178
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT16508479.03871501909366TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT245010.13414783846280956TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1917400.067.9395446
AGTCACG1944000.067.9247528
ACACGTC1964150.067.860213
GTATGCC1918400.067.83588447
GCACACG1976050.067.7999611
TATGCCG1915200.067.7703348
GTCACGT1933500.067.7371629
CACACGT1978950.067.6832912
ATGCCGT1916750.067.66093449
CTCGTAT1907000.067.6591144
TCGTATG1921700.067.6549445
ACGTCTG1962800.067.6542515
CACGTCT1966500.067.6515314
TGCCGTC1918700.067.5394750
GATCTCG1878350.067.46416541
GCCGTCT1908800.067.4453351
CGTCTGA1969650.067.4084916
CAGTCAC1961200.067.4066527
CGATCTC1844200.067.350840
ACGATCT1836800.067.3393339
Produced by FastQC (version 0.11.5)