FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20546453
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT234725111.42411782705268TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT217710.10595989487820598TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG2704050.067.74650615
CGTATGC2589350.067.661546
CACGTCT2710850.067.6586914
GCACACG2719900.067.645811
ACACGTC2717400.067.640813
GTATGCC2591000.067.6057647
CACACGT2721700.067.5742312
TATGCCG2570250.067.5516548
CACAGTC2642050.067.5235331
ATGCCGT2566900.067.500249
CGTCTGA2714450.067.4801616
GTCACAG2663400.067.45569629
CTCGTAT2581700.067.4334144
TGCCGTC2567200.067.4229550
TCGTATG2599850.067.3941145
CAGTCAC2703900.067.3476627
GCCGTCT2524550.067.2944851
ACTCCAG2704000.067.2575423
CCAGTCA2704300.067.2325226
GAACTCC2715800.067.2223321
Produced by FastQC (version 0.11.5)