FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18062029
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT5971953.3063561131476424TruSeq Adapter, Index 27 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182900.10126215609553056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG664500.066.4352445
CTCGTAT668950.066.2447944
CGTATGC676000.065.1545646
TCTCGTA682600.065.10943643
TCCTTTA713500.064.5662936
GTATGCC686650.064.3318747
TATCTCG705550.063.7765341
CCTTTAT723950.063.51307737
ATCTCGT710250.063.37368842
TATGCCG687500.063.1724548
TTATCTC710700.063.02541440
GCCGTCT667750.062.5599551
AGTCACA764150.062.34703428
GTCACAT759100.062.31418629
CCGTCTT674050.062.30250552
CATTCCT744850.062.08875733
ACATTCC751900.061.91986532
CTTTATC754500.060.92251638
CACATTC769550.060.8977531
CAGTCAC789650.060.86010427
Produced by FastQC (version 0.11.5)