FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21158631
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT5837412.7588788707549177TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259710.12274423614646902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG659400.064.9051145
CTCGTAT661200.064.51700644
CGTATGC668150.064.0341746
TCTCGTA678300.062.92574743
GTATGCC680050.062.9024747
TATGCCG687650.062.03931448
TATCTCG689300.061.9835241
GATATAT693350.061.6613137
TGATATA693000.061.62232236
TATATCT692250.061.48568739
GTCACAC707100.061.3779729
ATCTCGT699700.061.2468642
ATATATC695850.061.2183238
AGTCACA718650.061.04375528
GCCGTCT695400.060.95651651
CCGTCTT697400.060.89642352
CTGATAT706850.060.2880135
TCACACT717400.060.0815930
ACACTGA720200.059.61689832
CAGTCAC741450.059.57557727
Produced by FastQC (version 0.11.5)