FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868300
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5618463.540681736543927TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC541710.3413787236187871TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC714100.067.0009844
TATGCCG708400.066.6450446
GTATGCC721300.066.62794545
ATGCCGT671050.066.5305747
TGCCGTC661500.066.1991448
TCGTATG711300.066.1172743
GCCGTCT651250.065.968149
CTCGTAT656550.065.7578942
CCTTGTA790250.064.6032133
CCGTCTT673650.064.49168450
TGTAATC705850.064.25352536
GTAATCT689550.064.132937
TCTCGTA678100.063.95712741
GTCACCT810800.063.92738329
CACCTTG807400.063.8300131
ACCTTGT805300.063.6258132
AGTCACC823650.063.51244728
TAATCTC694500.063.42885638
ATCTCGT695200.062.87405840
CTGCTTG528600.062.73726357
Produced by FastQC (version 0.11.5)