FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17914012
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC4634482.587069831146702TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365230.20387951063111937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC538400.065.90140544
TATGCCG539800.065.6256546
GTATGCC543500.065.39873545
ATGCCGT540050.065.0699947
TGCCGTC538550.064.8860548
GCCGTCT534050.064.6602449
CGGCTAC544350.064.441233
CTCGTAT545250.064.374242
TCGTATG550900.064.3164443
ACGGCTA555750.063.65448832
GGCTACA550700.063.42448434
GCTACAT553300.062.89186535
CACGGCT565450.062.78040331
CCGTCTT553600.062.45266750
CTACATC556500.061.9627836
TCACGGC576900.061.8451930
CATCTCG563000.061.5760339
CTGCTTG515750.061.4673657
GTCACGG586350.061.3202229
TCTCGTA570750.061.22844341
Produced by FastQC (version 0.11.5)