FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18941369
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3009281.5887341617176667TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC431990.22806693645005277TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC395750.064.441744
TATGCCG396800.064.2265846
GTATGCC410700.063.97066545
ATGCCGT326400.062.53145247
TAGCTTA583300.062.41866734
ACTAGCT588500.062.30594632
CACTAGC592150.062.24879531
GCTTATC574100.062.11142736
CTCGTAT381000.062.04147742
TCGTATG367600.061.94093343
TGCCGTC324350.061.64137348
GCCGTCT315050.061.4732649
CTAGCTT593850.061.3916433
CTGCGTG223800.061.08899357
AGCTTAT606400.059.86008535
TCACTAG620350.059.6626430
CTTATCT474400.059.56078337
TGCGTGA211900.058.0106458
CCGTCTT349200.057.95740550
TATCTCG469350.057.8593239
Produced by FastQC (version 0.11.5)