FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19007906
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC261818513.774189539868306TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC541460.28486041545028684TruSeq Adapter, Index 9 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433660.22814717202410406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2914800.068.520943
CTCGTAT2841200.068.4443742
CGTATGC2918750.068.3670844
GTATGCC2921800.068.25497445
TATGCCG2927050.068.1577946
AGATCTC2824500.067.99094438
AGTCACG2963800.067.9903128
TCTCGTA2846250.067.9812841
GCCGTCT2911600.067.9643949
GTCACGA2951500.067.84730529
CCGTCTT2916500.067.8277650
CAGATCT2826900.067.80179637
ATGCCGT2934600.067.7590647
ATCTCGT2842100.067.7137740
GATCTCG2843450.067.6906239
CAGTCAC2987850.067.6329427
ACGTCTG3007650.067.5796115
TCCAGTC2986250.067.53458425
TCACGAT2959350.067.49802430
ACTCCAG2990350.067.483123
Produced by FastQC (version 0.11.5)