FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705645
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC8507765.092745595874927TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1327670.794743333765323TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC885410.5300064738595847TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC692630.41460835543913455TruSeq Adapter, Index 8 (97% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG667210.39939194206509243No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC448090.26822669822087086TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC388240.2324004849857638TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG288530.17271407359608085TruSeq Adapter, Index 8 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1070250.067.4517244
CTCGTAT1004550.067.44887542
TCGTATG961150.067.4388743
GTATGCC1101100.067.24905445
GTCACAC1878600.067.059129
AGTCACA1895550.066.8564728
TATGCCG1068650.066.6605346
CAGTCAC1913950.066.6192627
ACACTTG1869650.066.61536432
CCAGTCA1914800.066.4739226
TCTCGTA1028100.066.3840541
TCCAGTC1917900.066.3513225
ACTCCAG1921450.066.27898423
TCACACT1893150.066.2713330
GAACTCC1927300.066.2469921
ACGTCTG1935150.066.21265415
AATCTCG1271900.066.17248539
CACTTGA1875850.066.16048433
GAATCTC1278150.066.1260838
CGTCTGA1938600.066.1018416
Produced by FastQC (version 0.11.5)