FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17639855
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC13036707.390480250546277TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC1164430.6601131358506065TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC576880.32703216664762835TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC522000.2959207998024927TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGC338260.1917589458643509TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG335240.1900469136509342TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC250310.1419002593842183TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1601650.067.8556144
GTATGCC1621850.067.8192945
TATGCCG1586350.067.7569946
CTCGTAT1537100.067.6115242
TCGTATG1516950.067.5969743
ATGCCGT1359700.067.14178547
AGTCACC2238900.067.0245128
ACCAGAT2212550.066.8475232
GAACTCC2252700.066.82001521
ACTCCAG2247850.066.7951523
TCCAGTC2254950.066.71225
CATCTCG1768250.066.7095239
CAGTCAC2267800.066.6122827
TCTCGTA1567000.066.60116641
CCAGTCA2259850.066.5916426
CAGATCA2185850.066.5871434
CCAGATC2196700.066.5630633
TCACCAG2246000.066.52663430
AGATCAT2179050.066.50004635
CACCAGA2236850.066.4832931
Produced by FastQC (version 0.11.5)