FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19533273
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC12694566.4989415752290975TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1410500.067.8217744
GTATGCC1415350.067.6806945
TATGCCG1413100.067.5703346
ATGCCGT1397700.067.4198847
TCGTATG1420100.067.293643
CTCGTAT1414750.067.0932542
TGCCGTC1402500.066.65446548
GTCACGC1454700.066.4653329
GCCGTCT1397250.066.3164849
TCACGCC1444450.066.2235230
AGTCACG1482250.066.0401428
GCCAATA1435750.065.9497234
CCGTCTT1406350.065.9125950
CACGCCA1451550.065.8701531
TATCTCG1445100.065.7757239
TCTCGTA1447200.065.7657241
CTGCTTG1269950.065.6980757
ACGCCAA1451100.065.6685532
CGCCAAT1445400.065.6007733
ATCTCGT1466850.064.99013540
Produced by FastQC (version 0.11.5)