FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19958861
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10281925.151556494130602TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC7902763.9595245440108027TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC1317060.659887355295475TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC700370.35090679773760636TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATGC299180.14989833337683947TruSeq Adapter, Index 5 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATGC288860.14472769763765578TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC258750.12964166642575445TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATTC240310.12040266225612775TruSeq Adapter, Index 5 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATTC234560.11752173633555543TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2218500.068.1962144
TATGCCG2184000.068.043946
GTATGCC2234150.068.0178745
TCGTATG2018300.067.8654343
GCCGTCT1856550.067.5885549
ATGCCGT1957000.067.5790147
CTCGTAT1191450.067.50957542
GATCTCG1276000.067.3849839
TGCCGTC1912550.067.35638448
CCGTCTT1914700.067.04912650
CTGCGTG892950.066.8982357
CACAGTG2682350.066.7466833
TGCGTGA845050.066.7055358
AGTGATC1424500.066.68339536
GTCACAC2716000.066.5435629
TCTCGTA1218150.066.52705441
GCGTGAA875550.066.485459
CAGTCAC2752700.066.424127
ACACAGT2705650.066.3557232
CACACAG2717250.066.2892231
Produced by FastQC (version 0.11.5)