FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15673154
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC11388937.266520829183457TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC323700.2065314996585882TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1361800.066.6851244
TATGCCG1361550.066.5763546
GTATGCC1366050.066.5545145
CTCGTAT1323650.066.33790642
TCGTATG1371500.066.3119543
GCCGTCT1335000.066.2756749
ATGCCGT1354800.066.23340647
TGCCGTC1349050.066.1053948
CCGTCTT1349750.065.5903150
TCTCGTA1337250.065.412241
CTGCTTG1250900.065.268357
ACTGACC1377500.065.20998432
GACCAAT1358000.065.0470535
CCAATCT1320100.065.0317737
GTCACTG1417750.064.90517429
CTGACCA1377700.064.89435633
CAGTCAC1431150.064.8737127
ATCTCGT1345500.064.8361140
ACCAATC1320200.064.7998736
CCAGTCA1430400.064.6619726
Produced by FastQC (version 0.11.5)