Basic Statistics
Measure | Value |
---|---|
Filename | H7LNCBGXH_n01_AKM146.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14924671 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC | 1093745 | 7.328436251626585 | TruSeq Adapter, Index 3 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC | 61048 | 0.40904084250835415 | TruSeq Adapter, Index 3 (98% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 30100 | 0.20167948760813556 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC | 25116 | 0.16828511663674195 | TruSeq Adapter, Index 3 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC | 24707 | 0.16554468771874434 | TruSeq Adapter, Index 3 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC | 17265 | 0.11568094197855351 | TruSeq Adapter, Index 3 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC | 16629 | 0.11141954150949124 | TruSeq Adapter, Index 3 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 126745 | 0.0 | 67.909164 | 44 |
GTATGCC | 127895 | 0.0 | 67.77721 | 45 |
CTCGTAT | 121310 | 0.0 | 67.63016 | 42 |
TCGTATG | 120830 | 0.0 | 67.587746 | 43 |
TATGCCG | 125350 | 0.0 | 67.54251 | 46 |
GCCGTCT | 111240 | 0.0 | 67.14469 | 49 |
AGGCATC | 145150 | 0.0 | 67.04202 | 36 |
ATGCCGT | 117425 | 0.0 | 66.964455 | 47 |
GGCATCT | 131880 | 0.0 | 66.95753 | 37 |
GCATCTC | 131120 | 0.0 | 66.8836 | 38 |
TCTCGTA | 123460 | 0.0 | 66.84079 | 41 |
TGCCGTC | 114665 | 0.0 | 66.77484 | 48 |
CCGTCTT | 114235 | 0.0 | 66.689186 | 50 |
ACTTAGG | 155970 | 0.0 | 66.55678 | 32 |
CACTTAG | 156615 | 0.0 | 66.50615 | 31 |
CATCTCG | 131320 | 0.0 | 66.3604 | 39 |
ATCTCGT | 127410 | 0.0 | 66.18757 | 40 |
TTAGGCA | 155240 | 0.0 | 66.13674 | 34 |
TAGGCAT | 153745 | 0.0 | 66.12536 | 35 |
CTTCTGC | 120695 | 0.0 | 65.427155 | 54 |