FastQCFastQC Report
Sat 12 Dec 2020
H7LNCBGXH_n01_AKM146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7LNCBGXH_n01_AKM146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14924671
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC10937457.328436251626585TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC610480.40904084250835415TruSeq Adapter, Index 3 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301000.20167948760813556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC251160.16828511663674195TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC247070.16554468771874434TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC172650.11568094197855351TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC166290.11141954150949124TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1267450.067.90916444
GTATGCC1278950.067.7772145
CTCGTAT1213100.067.6301642
TCGTATG1208300.067.58774643
TATGCCG1253500.067.5425146
GCCGTCT1112400.067.1446949
AGGCATC1451500.067.0420236
ATGCCGT1174250.066.96445547
GGCATCT1318800.066.9575337
GCATCTC1311200.066.883638
TCTCGTA1234600.066.8407941
TGCCGTC1146650.066.7748448
CCGTCTT1142350.066.68918650
ACTTAGG1559700.066.5567832
CACTTAG1566150.066.5061531
CATCTCG1313200.066.360439
ATCTCGT1274100.066.1875740
TTAGGCA1552400.066.1367434
TAGGCAT1537450.066.1253635
CTTCTGC1206950.065.42715554