H7LNCBGXH_n01_AKM145.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H7LNCBGXH_n01_AKM145.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17839347
Sequences flagged as poor quality	0
Sequence length	76
%GC	40

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	1878745	10.531467323327474	TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC	119106	0.6676589675619853	TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC	53113	0.2977295077000296	TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC	41013	0.2299019128895245	TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC	28374	0.1590529070374605	TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC	26221	0.146984079630269	TruSeq Adapter, Index 2 (97% over 45bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	214940	68.32767	44
GTATGCC	216840	68.28902	45
TCGTATG	204780	68.09987	43
TATGCCG	212580	68.09494	46
CTCGTAT	208235	68.08502	42
GCCGTCT	184210	67.84624	49
ATGCCGT	193505	67.75175	47
GTATCTC	225175	67.66489	38
TGCCGTC	189660	67.528755	48
TCTCGTA	211085	67.52407	41
CCGTCTT	188895	67.4067	50
CGATGTA	255605	67.2663	34
TATCTCG	225815	67.16994	39
CTGCGTG	82360	67.1556	57
CCGATGT	256715	67.080246	33
ATCTCGT	217580	67.04746	40
TGTATCT	229785	67.0185	37
TGCGTGA	77850	66.92272	58
ATGTATC	253095	66.91727	36
GATGTAT	256930	66.84472	35